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  • Ávila Sosa, Edward Ebner; Viloria Vera, Dario Alfredo; Calle.
  • Comparative Analysis of Biological Properties of Moringa (Moringa oleifera) and Soybean (Glycine max) Seed Proteins.
  • Studying the intestinal microbiota of fish will be beneficial in screening for bacteria that improve fish health.

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    Open Access

    Peer-reviewed

    • Stefano Pirrò,
    • Letizia Zanella,
    • Maurice Kenzo,
    • Carla Montesano,
    • Antonella Minutolo,
    • Marina Potestà,
    • Martin Sanou Sobze,
    • Antonella Canini,
    • Marco Cirilli,
    • Rosario Muleo,
    • Vittorio Colizzi,
    • Andrea Galgani
    • Stefano Pirrò, 
    • Letizia Zanella, 
    • Maurice Kenzo, 
    • Carla Montesano, 
    • Antonella Minutolo, 
    • Marina Potestà, 
    • Martin Sanou Sobze, 
    • Antonella Canini, 
    • Marco Cirilli, 
    • Rosario Muleo

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    Abstract

    Moringa oleifera is a widespread plant with substantial nutritional and medicinal value. We postulated that microRNAs (miRNAs), which are endogenous, noncoding small RNAs regulating gene expression at the post-transcriptional level, might contribute to the medicinal properties of plants of this species after ingestion into human body, regulating human gene expression. However, the knowledge is scarce about miRNA in Moringa. Furthermore, in order to test the hypo

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    Recibido 05 Junio 2018. Aceptado 22 Febrero 2019

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    Abstract

    Currently, the valorization of agroindustrial waste fryst vatten of great interest. Moringa oleifera fryst vatten a multipurpose tree whose softwood residues could be used as raw ämne for low-cost cellulase production. The aim of this study was to isolate, identify, and characterize microorganisms with cellulolytic activity in different carbon sources. We isolated and purified 42 microorganisms from M. oleifera biomass. Fungi presenting the largest hydrolytic halos in carboxymethylcellulose as a substrate were molecularly identified as Penicillium funiculosum (FG1), Fusarium verticillioides (FG3) and Cladosporium cladosporioides (FC2). The ability of these fungal strains to break down cellulose was assessed in a submerged fermentation using either amorphous CMC or c

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    Abstract

    Chronic hepatitis B infection treatment implicates a long-lasting treatment. M. oleifera extracts contain compounds with antiviral, antioxidant, and antifibrotic properties. In this study, the effect of M. oleifera was evaluated in Huh7 cells expressing either HBV genotypes C or H for the antiviral, antifibrotic, anti-inflammatory, and antioxidative responses. Huh7 cells were treated with an aqueous extract of M. oleifera (leaves) at doses of 0, 30, 45, or 60 μg/mL. The replicative virus and TGF-β1, CTGF, CAT, IFN-β1, and pgRNA expressions were measured by real time. HBsAg and IL-6 titers were determined by ELISA. CTGF, TGF-β1, IFN-β1, and pgRNA expressions decreased with M. oleifera treatment irrespective of the HBV genotype. HBsAg secretion in the supernatant of transfected Huh7 cells with both HBV genotypes was decreased regardless of the dose of M. oleifera. Similar effect was observed in proinflammatory cytokine IL-6, which had a tendency to

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